Micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and submergence tolerance, was. Altererythrobacter indicus is a gramnegative, rodshaped and nonsporeforming bacterium from the genus of altererythrobacter which has been isolated from the rhizosphere from the rice plant porteresia coarctata in pichavaram in india references. Current affairs daily digest april 21 2019 day today gk. Functional screening of genes from a halophyte wild rice. Antifeeding and toxic effects of chemical extracts from porteresia coarctata takeoka on the insect pest spodoptera litura f. We sequenced the transcriptome of porteresia under different conditions using illumina platform and generated about 375 million high. Pdf deep transcriptome sequencing of wild halophyte rice. Mangroves are characterized by alkaline soils in which the halophytic wild rice relative porteresia coarctata thrives. On the basis of 16s rrna gene sequence similarities, strain mssrf26t was shown to belong to the alphaproteobacteria, most closely related to altererythrobacter luteolus and altererythrobacter epoxidivorans 96. Functional screening of genes from a halophyte wild rice relative. Recalcitrant seed storage physiology in three aquatic. Regulatory region of ubiquitin 2 gene from porteresia.
Usda, ars, germplasm resources information network. The experimental approach was based on activation tagging without regeneration of plants for the identification. In the present study, we overexpressed pcino1 and pcimt1 genes, earlier characterized in this laboratory from wild halophytic rice porteresia coarctata, into ir64 indica rice either singly or in. Genetic relationship of porteresia coarctata tateoka using molecular. Leaf extracts of vernonia cinerea cause reduction in population and larval mortality in culex quinquefasciatus arivoli et al. Introgression of a novel salttolerant lmyo synthase from. Tateoka pcino1, a halophytic wild rice, that codes for a unique salttolerant mips enzyme. Researchers have developed a new salttolerant transgenic rice plant by overexpressing a gene from wild rice called porteresia coarctata into the commonly used ir 64 indicia rice variety. Draft genome sequence of first monocothalophytic species. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Our previous work on a salttolerant mips from porteresia coarctata pcino1 demonstrated that it is a variant of the osino1 gene isolated from the domesticated rice oryza sativa, being different in a 37amino acid residue stretch residues trp174 to ser210, and can characteristically synthesize the osmolyte inositol even under salt stress.
A gramnegative, rodshaped, nonsporeforming organism, strain mssrf26t, was isolated from mangroveassociated wild rice in india. The journal covers all branches of pureapplied science and technology such as physics, chemistry, life sciences, medicine, earth sciences, engineering and technology. Pdf distribution of arsenic in the estuarine ecosystem. Process for the isolation of pharmaceutical compound. Nac proteins nam no apical meristem, ataf arabidopsis transcription activation factor and cuc cupshaped cotyledon are plantspecific transcription factors reported to be involved in regulating growth, development and stress responses. These strains were determined to be nitrogenfixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. Draft genome sequence of first monocothalophytic species oryza. In higher plants, most mirnas regulate their targets via cleavage, which normally occurs between the tenth and eleventh nucleotides of the complementary region between the mirna and the mrna target. While members of the genus oryza are very sensitive to salinity, salt concentrations as high as 20% of that of seawater had no adverse effect on the growth of the tropical wild rice porteresia coarctata tateoka in experiments undertaken in a greenhouse in the uk. The corresponding homologue, termed osino1 from the cultivated rice, oryza sativa, however, codes for a saltsensitive mips protein 14. Muthiah was born in 1930 in the erstwhile madras presidency, in what is today the ramanathapuram district of tamil nadu. In vitro propagation of salttolerant wild rice relative, porteresia. Regulatory region of ubiquitin 2 gene from porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots, plant cell reports on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. A gene putatively identified as the archaeoglobus fulgidus inositol1phosphate synthase ips gene was overexpressed to high level about 30.
Other authors maintain instead that it should belong in. Major beds along the mandovi estuary of goa were monitored for their various ecological and environmental parameters. Pollen tube growth in crosses between porteresia coarctata. Cloning, characterization and expression of a chloroplastic. Porteresia coarctata is a wild relative of rice with capability of high salinity and submergence tolerance.
A novel salttolerant lmyoinositol1phosphate synthase from porteresia coarctata roxb. Identification of soybean seed developmental stage. Comparative study with fluorescence microscopy revealed that the pollen tubes of the selfpollinated rice variety br9 reached the ovary within 75 min after pollination. The 3 cleavage fragments contain both a free 5 monophosphate and a 3 polya tail. The transcriptome analyses of porteresia can lead to the identification of candidate genes involved in salinity and submergence tolerance. Using illumina and nanopore reads, we achieved the assembled genome. Characterization of progenies from intergeneric hybridization. Porteresia coarctata is considered by some botanists to be the only species in the genus porteresia. Download fulltext pdf deep transcriptome sequencing of wild halophyte rice, porteresia coarctata, provides novel insights into the salinity and submergence tolerance factors. Three experimental feeds were formulat ed by reducing fishmeal at a level of 5%, incorporated with selected ingredients like green seaweed, enteromorpha intestinalis referred as ent feed. Total arsenic content in the sediment and in selected estuarine animal. A concentration of 1,000 and 2,000 ppm extract exhibited significant. The study of tobacco promotes the understanding about the important adjustment for survival in high salinity environments, including cellular ion transport, osmotic regulation, antioxidation.
A cdna for porteresia coarctata inositol methyl transferase 1 pcimt1, coding for. Recalcitrant seed storage physiology in three aquatic grasses zizania palustris, spartina anglica and porteresia coarctata r. The board of trustees of the royal botanic gardens, kew. Porteresia coarctata is a native of india, sri lanka, bangladesh and myanmar and is grown mainly in saline estuaries. Porteresia coarctata is a species of grass in the family poaceae, native to india, sri lanka. Porteresia coarctata, forms an important constituent in the plant succession leading to formation of mangrove communities along the estuaries in india. Selective manipulation of the inositol metabolic pathway. Research journal of animal, veterinary and fishery. Pdf in vitro propagation of salttolerant wild rice relative. Salt tolerance mechanisms in salt tolerant grasses stgs and their prospects in cereal crop improvement. Deep transcriptome sequencing of wild halophyte rice. It is saline tolerant and can tolerate water logging also.
We evaluated stored carbon in the above ground biomass agb and below ground biomass bgb of porteresia coarctata for 12 consecutive years 20012012 along the hooghly estuary in the north. Porteresia coarctata, the only halophyte wild rice, is a rich source of genes and promoters for improving salt tolerance in rice full length cdna overexpression foxcox system was used to identify porteresia coarctata genes with positive involvement in salt stress response using arabidopsis model. Pdf cloning, characterization and expression of a chloroplastic. Two facultatively anaerobic, nitrogenfixing bacteria strains mssrf30t and mssrf31 were isolated from a mangroveassociated wild rice porteresia coarctata tateoka. Advances in salt tolerance molecular mechanism in tobacco. Effect of storage temperature and moisture on the germination of papaya seeds. Porteresia coarctata, the only halophyte wild rice, is a rich source of genes and promoters for improving salt tolerance in rice. Ecological differentiation involving cycles of hybridization, differentiation and selection were enhanced when ancestral forms of o. We report for the first time the draft genome with the coverage of 85. Process for the isolation of pharmaceutical compound cyclosporin a from fungus fusarium nivale. Tasmia islam department of biochemistry and molecular biology, university of dhaka, dhaka. Download the understanding the publishing process pdf. Master chronicler of madras s muthiah passed away renowned citybased chronicler and writerjournalist s muthiah passed away in chennai.
Salinity responsive transcriptome profiling in a set of contrasting finger millet genotypes through rnasequencing resulted in the identification of a. Determining genetic diversity among porteresia coarctata tateoka accessions. Genomewide screening of salt tolerant genes by activation. Characterization of porteresia coarctata beds along the. Seed developmental stagespecific library construction, sequencing and sequence analysis. Germplasm accessions belonging to six different wild rice taxaoryza meyeriana var. The best population is in sundarbans of bengal and bhitarkanika of orrisa. Nitrate and nitrite values in the over lying waters of beds varied from 0. Tateoka, a halophytic wild rice molecular cloning, bacterial overexpression, characterization, and functional introgression into tobaccoconferring salt tolerance phenotype. Proteomics, metabolomics, and ionomics perspectives of.
Genbank sequence accessions of two plastid dna regions rbcl and matk. Soil salinity is an increasing threat to crop production under climatic uncertainties. Salt tolerance in the halophytic wild rice, porteresia. Tobacco, an economic crop and important model plant, has received more progress in salt tolerance with the aid of transgenic technique. The recombinant protein was purified to homogeneity by heat treatment followed by two column chromatographic steps. Micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and submergence. Full length cdna overexpression foxcox system was used to identify porteresia coarctata genes with positive involvement in salt stress response using arabidopsis model. Zizania palustris, spartina angelica and porteresia coarctata adapted. Oryza coarctata plant, collected from sundarban delta of west bengal, india, has been used in the present study to generate draft genome sequences, employing the hybrid genome assembly with illumina reads and third generation oxford nanopore sequencing technology. Inositol1phosphate synthase from archaeoglobus fulgidus. Functional screening of genes from a halophyte wild rice relative porteresia coarctata in arabidopsis model identifies candidate genes involved in salt tolerance open access. A novel salttolerant lmyoinositol1phosphate synthase. The possible overlap between salinity and mechanical challenges in. Salt tolerance mechanisms in salt tolerant grasses stgs.
Porteresia coarctata, the only halophyte wild rice, is a rich source of genes and. Salinity represents a major abiotic stress factor that can adversely limit the production, quality and geographical distribution of crops. Pdf approaches to breeding for salinity tolerance a. In this study we focused on dedifferentiated calli with fundamental cell functions, the salt tolerance of which had not been previously examined. Oryza coarctata in world checklist of selected plant families. Current science is a fortnightly journal published since 1932 by the current science association, bangalore india in collaboration with the indian academy of sciences. Micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and. Phylogeny of rice genomes with emphasis on origins of. Approaches to breeding for salinity tolerance a case study on porteresia coarctata. Porteresia coarctata is a species of grass in the family poaceae, native to india, sri lanka, bangladesh, and myanmar. In this study, they have found a heightened level of proteins involved in protection and stabilization of psi and psii under salt stress which involved oxygen evolving complex oec. Porteresia coarctata 1 rhynchoryza subulata 1 total 80647 most samples in the collection are landrace varieties of o. Pdf in vitro propagation of salttolerant wild rice. Salt stress has become a key research field in abiotic stress.